The 3D-SIM microscope was acquired using funds from the Department of Cell Biology, the Office of Tools and Technology, and the Laboratory of Systems Pharmacology. It is available for use by labs whose Principal Investigator has a primary appointment in one of the HMS basic science departments and LSP.
170nm fluorescent beads imaged with widefield (WF) and structured illumination (SIM)
Sub-second 3D stacks with structured illumination increasing lateral resolution, axial resolution and contrast
Galvos deliver TIRF illumination in a ring in the back focal plane, greatly decreasing interference fringing
Three cameras for simultaneous multicolor acquisition, enabling high temporal resolution in multi-channel live cell experiments
405, 445, 488, 514, 568, & 642nm laser lines, each 100mW
Three sCMOS cameras providing high speed and low median read noise
Hardware focus maintenance prevents thermal focus drift for live cell imaging
For more detailed information, view the microscope technical specifications
For important sample prep information, see our sample prep guidelines
For questions, please email Talley Lambert
If you want to be trained or if you want to come in for a free consultation to discuss whether SIM might benefit your experiment, please fill out a training request
